New protein scaffolds for assembling multi-enzyme systems with unprecedented control

Cell-free biocatalysis is being increasingly used as a substitute for conventional chemical catalysts, given that enzymes (biological catalysts) are more sustainable and selective in the manufacture of valuable chemicals. Chemical biomanufacturing has benefited from extraordinary advances in molecular and synthetic biology, which have stimulated the creation of new enzymatic cascades (sequences of reactions in which each newly formed product is subsequently transformed into the next).